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Ellenberger and co-staff (eight) had intuited particularly such a motion of your OB domain every time they solved the crystal composition in the much larger human DNA ligase I (HuLig1; 919 amino acids) bound to an adenylylated nick. Their landmark construction was the 1st to document the circumferential envelopment of your DNA duplex by a ligase clamp plus the ensuing bending and distortion on the duplex that drive The bottom pairs over the 3′-OH aspect with the nick into an RNA-like A helical conformation (eight). Comparable distortions ended up noticed inside the ChVLig·DNA intricate (7).
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